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CRISPR / Cas9系統(tǒng)是用于基因編輯的RNA引導核酸內(nèi)切酶技術(shù)。該系統(tǒng)需要包含與PAM（Protospacer Adjacent Motif）位點相鄰的20bp靶序列的指導RNA（gRNA），以將Cas9核酸內(nèi)切酶引導至切割位點。Geneious中的Find CRISPR網(wǎng)站工具將搜索所選基因中的gRNA（“CRISPR”）位點，并在您感興趣的基因組中搜索脫靶結(jié)合位點。選定序列中的每個CRISPR站點根據(jù)它可能會綁定到多少個脫離目標站點以及脫離目標站點與原始序列的相似程度進行評分。
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