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CRISPR / Cas9系統是用于基因編輯的RNA引導核酸內切酶技術。該系統需要包含與PAM（Protospacer Adjacent Motif）位點相鄰的20bp靶序列的指導RNA（gRNA），以將Cas9核酸內切酶引導至切割位點。Geneious中的Find CRISPR網站工具將搜索所選基因中的gRNA（“CRISPR”）位點，并在您感興趣的基因組中搜索脫靶結合位點。選定序列中的每個CRISPR站點根據它可能會綁定到多少個脫離目標站點以及脫離目標站點與原始序列的相似程度進行評分。
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